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KMID : 1098420060140030178
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Korean Journal of Medicinal Crop Science
2006 Volume.14 No. 3 p.178 ~ p.182
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Characterization of 18S rDNA in Polygonatum spp. Collections
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Yun Jong-Sun
Kim Ik-Hwan
Park Jae-Seong
Lee Cheol-Hee
Hong Eui-Yon
Yun Tae
Jong Seung-Keun
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Abstract
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This study was conducted to investigate the variation in sequence, the base composition and the sequence similarity of 18S rDNA (18S ribosomal RNA coding region) in the 10 Polygonatum spp. collections. The entire 18S rDNA region of 10 Polygonatum spp. collections ranged from 913 bp to 914 bp. There were 8 variable sites in the entire 18S region, and they were attributable to nucleotide substitution and deletion. T{rightarrow}C transition happened in 4 sites, and A{rightarrow}G transition happened in 1 site. C{rightarrow}A transversion happened in 1 site, and deletion happened in 2 sites. Transition rates were five times that of transversion. Base compositions of 18S rDNA were 23.09{sim}23.33% in adenine, 23.33{sim}23.52% in guanine, 25.60{sim}25.85% in thymine and 27.38{sim}27.79% in cytosine. The A + T content of 18S rDNA of 10 Polygonatum spp. collections averages 48.99%, ranging from 48.80% to 49.18%, and the G + C content averages 51.01%, ranging from 50.82% to 51.20%. Pairwise sequence comparisons indicated that 18S rDNA sequence similarity ranged from 99.7% to 100%.
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KEYWORD
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18S rDNA, nucleotide sequence, Polygonatum
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